Mandelonitrile lyase

mandelonitrile lyase
Identifiers
EC number	4.1.2.10
CAS number	Template:CAS
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / EGO
Search PMC articles PubMed articles NCBI proteins

File:Mandelonitrile lyase.png

Model of mandelonitrile lyase based on PDB entry 1JU2

In enzymology, a mandelonitrile lyase (EC 4.1.2.10, (R)-HNL, (R)-oxynitrilase, (R)-hydroxynitrile lyase) is an enzyme that catalyzes the chemical reaction

mandelonitrile $\rightleftharpoons$ hydrogen cyanide + benzaldehyde

Hence, this enzyme has one substrate, mandelonitrile, and two products, hydrogen cyanide and benzaldehyde.

This enzyme belongs to the family of lyases, specifically the aldehyde-lyases, which cleave carbon-carbon bonds. The systematic name of this enzyme class is mandelonitrile benzaldehyde-lyase (hydrogen cyanide-forming). Other names in common use include hydroxynitrile lyase, (R)-oxynitrilase, oxynitrilase, D-oxynitrilase, D-alpha-hydroxynitrile lyase, and mandelonitrile benzaldehyde-lyase. This enzyme participates in cyanoamino acid metabolism. It has 2 cofactors: flavin, and flavoprotein.

Structural studies

As of late 2007, only one structure has been solved for this class of enzymes, with the PDB accession code 1JU2.