Serratia marcescens

Serratia marcescens
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S. marcescens on an XLD agar plate
Scientific classification
Domain:	Bacteria
Phylum:	Proteobacteria
Class:	Gamma Proteobacteria
Order:	Enterobacteriales
Family:	Enterobacteriaceae
Genus:	Serratia
Species:	S. marcescens
Binomial name
Serratia marcescens Bizio 1819

Serratia marcescens is a species of rod-shaped gram-negative bacteria in the family Enterobacteriaceae. A human pathogen, S. marcescens is involved in hospital-acquired infections (HAIs), particularly catheter-associated bacteremia, urinary tract infections and wound infections, and is responsible for 1.4% of HAI cases in the United States. It is commonly found in the respiratory and urinary tracts of hospitalized adults and in the gastrointestinal system of children. Due to its abundant presence in the environment, and its preference for damp conditions, S. marcescens is commonly found growing in bathrooms (especially on tile grout, shower corners, toilet water line, and basin), where it manifests as a pink, pink-orange, or orange discoloration and slimy film feeding off phosphorus-containing materials or fatty substances such as soap and shampoo residue.

Once established, complete eradication of the organism is often difficult, but can be accomplished by application of a bleach-based disinfectant. Rinsing and drying surfaces after use can also prevent the establishment of the bacterium by removing its food source and making the environment less hospitable.

S. marcescens may also be found in environments such as dirt, supposedly "sterile" places, and the subgingival biofilm of teeth. Due to this, and because S. marcescens produces a reddish-orange tripyrrole pigment called prodigiosin, it may cause staining of the teeth. The biochemical pathway for the production of prodigiosin by S. marcescens is unknown except for the final two steps. In these steps, a monopyrrole and a bipyrrole undergo a condensation reaction by way of an enzyme to form prodigiosin.

Identification

S. marcescens is a motile organism and can grow in temperatures ranging from 5–40°C and in pH levels ranging from 5 to 9. It is differentiated from other Gram-negative bacteria by its ability to perform casein hydrolysis, which allows it to produce extracellular metalloproteinases which are believed to function in cell-to-extracellular matrix interactions. S. marcescens also exhibits tryptophan and citrate degradation. One of the end products of tryptophan degradation is pyruvic acid, which is then incorporated into different metabolic processes of S. marcescens. A final product of citrate degradation is carbon. Thus, S. marcescens can rely on citrate as a carbon source. In identifying the organism, one may also perform a methyl red test, which determines if a microorganism performs mixed-acid fermentation. S. marcescens results in a negative test. Another determination of S. marcescens is its capability to produce lactic acid by oxidative and fermentative metabolism. Therefore, it is said that S. marcescens is lactic acid O/F+.

Test	Result
Gram stain	-
Oxidase	-
Indole production	-
Methyl Red	>70% -
Voges-Proskaeur	+
Citrate (Simmons)	+
Hydrogen sulfide production	-
Urea hydrolysis	>70% -
Phenylalanine deaminase	-
Lysine decarboxylase	+
Motility	+
Gelatin hydrolysis, 22°C	+
Acid from lactose	-
Acid from glucose	+
Acid from maltose	+
Acid from mannitol	+
Acid from sucrose	+
Nitrate reduction	+ (to nitrite)
Deoxyribonuclease, 25°C	+
Lipase	+
Pigment	some biovars produce red
Catalase production (24h)	+

Pathogenicity

File:Serratia marcescens - antibiogram.jpg

The antibiogram of S. marcescens on Mueller-Hinton agar.

In humans, S. marcescens can cause infection in several sites, including the urinary tract, respiratory tract, wounds, and the eye, where it may cause conjunctivitis, keratitis, endophthalmitis, and tear duct infections. It is also a rare cause of endocarditis and osteomyelitis (particularly in people who use intravenous drugs recreationally), pneumonia, and meningitis. Most S. marcescens strains are resistant to several antibiotics because of the presence of R-factors, which are a type of plasmid that carry one or more genes that encode resistance; all are considered intrinsically resistant to ampicillin, macrolides, and first-generation cephalosporins (such as cephalexin).

In elkhorn coral, S. marcescens is the cause of the disease known as white pox disease. In silkworms, it sometimes occurs as a secondary pathogen in viral flacherie disease.

In research laboratories employing Drosophila fruit flies, infection of them with S. marcescens is common. It manifests as a pink discolouration or plaque in or on larvae, pupae, or the usually starch and sugar-based food (especially when improperly prepared).

A rare clinical form of gastroenteritis occurring in early infancy caused by infection with S. marcescens. The red color of the diaper can be mistaken for hematuria (blood in the urine) which may cause unnecessary investigations by the physicians.

S. marcescens causes curcurbit yellow vine disease, leading to sometimes serious losses in melon fields.

History

Possible role in medieval miracles

File:Bloody bread - Serratia marcescens in action.JPG

"Bloody bread": S. marcescens growing on bread

Because of its red pigmentation, caused by expression of the pigment prodigiosin, and its ability to grow on bread, S. marcescens has been evoked as a naturalistic explanation of medieval accounts of the "miraculous" appearance of blood on the Corporal of Bolsena. This followed celebration of a Mass at Bolsena in 1263, led by a Bohemian priest who had doubts concerning transubstantiation, or the turning of bread and wine into the Body and Blood of Christ during the Mass. During the Mass, the Eucharist appeared to bleed and each time the priest wiped away the blood, more would appear. While Serratia possibly could generate a single appearance of red pigment, it is unclear how it could have generated more pigment after each wiping, leaving this proposed explanation open to doubt. This event is celebrated in a fresco in the Apostolic Palace in the Vatican City, painted by Raphael.

Discovery

S. marcescens was discovered in 1819 by Venetian pharmacist Bartolomeo Bizio, as the cause of an episode of blood-red discoloration of polenta in the city of Padua. Bizio named the organism four years later in honor of Serafino Serrati, a physicist who developed an early steamboat; the epithet marcescens (Latin for "decaying") was chosen because of the pigment's rapid deterioration (Bizio's observations led him to believe that the organism decayed into a mucilage-like substance upon reaching maturity). Serratia was later renamed Monas prodigiosus and Bacillus prodigiosus before Bizio's original name was restored in the 1920s.

Role in biowarfare testing

Until the 1950s, S. marcescens was erroneously believed to be a nonpathogenic "saprophyte", and its reddish coloration was used in school experiments to track infections. During the Cold War, it was used as a simulant in biological warfare testing by the U.S. military which studied it in field tests as a substitute for the tularemia bacterium, which was being weaponized at the time.

On 26 and 27 September 1950, the U.S. Navy conducted a secret experiment named "Operation Sea-Spray" in which some S. marcescens was released by bursting balloons of it over urban areas of the San Francisco Bay Area in California. Although the Navy later claimed the bacteria were harmless, beginning on September 29, 11 patients at a local hospital developed very rare, serious urinary tract infections, and one of these individuals, Edward J. Nevin, died. Cases of pneumonia in San Francisco also increased after S. marcescens was released. (That the simulant bacteria caused these infections and death has never been conclusively established. Nevin's son and grandson lost a lawsuit they brought against the government between 1981 and 1983.) The bacterium was also combined with phenol and an anthrax simulant and sprayed across south Dorset by US and UK military scientists as part of the DICE trials which ran from 1971 to 1975.

Since 1950, S. marcescens has steadily increased as a cause of human infection, with many strains resistant to multiple antibiotics. The first indications of problems with the influenza vaccine produced by Chiron Corporation in 2004 involved S. marcescens contamination.

Contaminated injectables

In early 2008, the U.S. Food and Drug Administration issued a nationwide recall of one lot of Pre-Filled Heparin Lock Flush Solution USP. The heparin IV flush syringes had been found to be contaminated with S. marcescens, which resulted in patient infections. The Centers for Disease Control and Prevention confirmed growth of S. marcescens from several unopened syringes of this product.

S. marcescens has also been linked to 19 cases in Alabama hospitals in 2011, including 10 deaths. All of the patients involved were receiving total parenteral nutrition at the time, and this is being investigated as a possible source of the outbreak.

See also

• Eucharistic miracle